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KMID : 1101220030350020174
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2003 Volume.35 No. 2 p.174 ~ p.181
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Comparative Analysis about CLO Test, Giemsa Stain and Immunohistochemical Stain of Method for H. pylori Detection
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Yu Suk
Back Oun-Chul
Kim Jong-Yull
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Abstract
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In order to detect a rational method for reducing the ratios of the false positive and the false negative appearing from the CLO test and giemsa staining used for the examination of H. pylori, we have compared with the results of CLO test and giemsa staining of which the false positive and the false negative are shown through this study. We have divided the groups as following four groups: A, B, C, and D group. And as for the respective group we have executed the immunohistochemical staining reported as the highest in terms of sensitiveness and particularity and have got to the following results through the reciprocal comparisons of H. pylori detection results. 1. A Group; CLO test positive and giemsa staining positive As a result, immunohistochemical staining showed a higher degree rather than giemsa staining in terms of sensitiveness. 2. B Group; CLO test negative and giemsa staining positive As a result, false positive of giemsa staining against the results of the negative of immunohistochemical staining was elaborated as stained in the mucus and the similar bacteria. And the false negative of CLO test that does not coincide with the results of immunohistochemical staining was elaborated as inadequacy of the option of the organic extraction. In such a way, immunohistochemical staining showed a higher degree rather than CLO test in terms of the sensitiveness and particularity. 3. C Group; CLO test positive and Giemsa staining negative As a result, false negative was judged as stained bacteria as the number of H. pylori is not so many or colored in blue color in the stain background in the giemsa staining. And the false positive of CLO test was elaborated as polluted from bacteria in the inside of the oral cavity at the time of organic extraction. 4. D Group; CLO test negative and giemsa staining negative Immunohistochemical staining showed negative in all for cases, and coincided with the results of CLO test and giemsa staining. In conclusion, immunohistochemical staining was made in only H. pylori through antigen and antibody. CLO test and giemsa staining showed positive and negative in gastric mucosa. Therefore, as for the detection body of which the results of CLO test and giemsa staining does not coincide like immunohistochemical staining, to let reconfirm through the examination method of high sensitiveness and high characteristics would be of help for an exact detection of H. pylori and an earlier treatment.
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KEYWORD
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H. pyrori, CLO test, Giemsa stain, Immunohistochemical stain
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